| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DARLING, Emily | FR | Marceline High School | 2:46.16 | Verified |
| 3 | WEIMER, Payton | FR | Macon High School | 2:48.54 | Verified |
| 4 | GITTEMEIER, Melia | FR | Northeast (Cairo) High School | 2:50.19 | Verified |
| 5 | WEAVER, MAGGIE | JR | Clark County High School | 2:50.79 | Verified |
| 6 | JOHNSON, Avery | SO | Monroe City High School | 2:51.62 | Verified |
| 7 | HAYS, Ella | JR | Monroe City High School | 2:54.57 | Verified |
| 9 | MILLER, Jaelyn | FR | Westran High School | 2:57.40 | Verified |
| 10 | UHLMEYER, Addy | SO | North Shelby High School | 2:58.05 | Verified |
| 11 | LITTLE, Tianna | FR | Clark County High School | 2:59.47 | Unverified: |
| 12 | BUCKMAN, Shelby | SO | South Shelby High School | 3:01.01 | Verified |
| 13 | MARTIE, Sophia | SO | Macon High School | 3:06.66 | Verified |
| 14 | THOROUGHMAN, Klaire | SO | Silex High School | 3:18.24 | Verified |
| 16 | ISOM, Maddison | JR | Silex High School | NT | Unverified: |
| 17 | PARSONS, Hadlee | FR | North Shelby High School | NT | Unverified: |
| 18 | LEE, Alyssa | FR | Marion County High School | NT | Unverified: |
| 19 | BOONE, Patience | FR | Atlanta High School | NT | Unverified: |