| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | MCDOWELL, Ericka | 9 | Clark County High School | NT | |
| 4 | ZAHRINGER, Chloi | FR | Knox County High School | 1:00.44 | Verified |
| 5 | GORDON, Liberty | FR | Northeast (Cairo) High School | 1:01.24 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MASSEY, Kiyah | FR | Westran High School | 59.13 | Verified |
| 2 | SCHWANKE, Mary | SR | North Shelby High School | 58.95 | Verified |
| 3 | NEVES, LUNDYN | JR | Clark County High School | 57.62 | Verified |
| 4 | FOSS, Gracee | SO | Monroe City High School | 54.39 | Verified |
| 5 | KINION, CLAIRE | SO | Silex High School | 56.39 | Verified |
| 6 | HATHAWAY, Kennedy | FR | Marion County High School | 58.51 | Verified |
| 7 | KIRBY, Ceairra | SR | North Shelby High School | 59.05 | Verified |
| 8 | STEPHENSON, Jill | FR | Westran High School | 59.97 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHRISTENSEN, Sylvie | JR | Atlanta High School | 53.87 | Verified |
| 2 | THOROUGHMAN, Rita | SR | Silex High School | 52.45 | Verified |
| 3 | MAGERS, Kyley | SR | Atlanta High School | 52.14 | Verified |
| 4 | YOUNGBLOOD, Audri | SO | Monroe City High School | 48.26 | Verified |
| 5 | MITCHELL, Kamryn | JR | South Shelby High School | 51.44 | Unverified: Palmyra Panther Open |
| 6 | HARMON, Macie | SO | Northeast (Cairo) High School | 52.33 | Verified |
| 7 | MCVICKER, Danni | SO | Macon High School | 53.55 | Verified |
| 8 | WEESE, Payton | JR | Marceline High School | 54.35 | Unverified: |