| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GARCIA, Marc | SO | Marceline High School | NT | Unverified: |
| 2 | NOXON, Joey | FR | Silex High School | NT | Unverified: |
| 3 | WOHLGEMUTH, Jackson | SO | Westran High School | 1:08.96 | Verified |
| 4 | CAMPBELL, Blake | FR | Knox County High School | 1:05.62 | Verified |
| 5 | MCELHANEY, Jacob | SR | Atlanta High School | 1:06.57 | Verified |
| 6 | CHIDESTER, Matthew | FR | Northeast (Cairo) High School | 1:16.67 | Verified |
| 7 | SALINAS, Victor | SR | Missouri Military Academy | NT | Unverified: |
| 8 | JONES, Riley | SO | Silex High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WATT, Angstrom | FR | Putnam County High School | 1:03.94 | Verified |
| 2 | QUINN, Jake | JR | Macon High School | 1:02.08 | Verified |
| 3 | HUNOLT, Alious | FR | Knox County High School | 58.66 | Verified |
| 4 | TULEY, Wyatt | FR | Marion County High School | 57.90 | Verified |
| 5 | MARTIN, Shawn | JR | Marion County High School | 58.52 | Verified |
| 6 | QUIROGA, Gerardo | SO | Missouri Military Academy | 59.77 | Verified |
| 7 | WINKLER, Nolen | SO | Westran High School | 1:03.55 | Verified |
| 8 | HETHERITON, Payton | FR | South Shelby High School | 1:04.20 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TOMLIN, Chase | SR | Putnam County High School | 57.01 | Unverified: |
| 2 | ELSEN, Preston | SO | South Shelby High School | 55.32 | Verified |
| 3 | GLOVER, Ethan | JR | Macon High School | 54.29 | Verified |
| 4 | WOOLEN, Gage | SO | Monroe City High School | 52.06 | Verified |
| 5 | HAMNER, KADEN | JR | Clark County High School | 52.76 | Unverified: |
| 6 | WHEELER, CALLAN | JR | Clark County High School | 54.96 | Verified |
| 7 | KARR, Lorenzo | SO | Monroe City High School | 56.68 | Verified |
| 8 | DEWEESE, Jack | JR | Marceline High School | 57.79 | Verified |