| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MYERS, Mylee | FR | Marion County High School | 1:29.40 | Verified |
| 2 | BECKER, Abigail | JR | Knox County High School | 1:22.52 | Verified |
| 3 | HUGHES, Kayla | FR | Northeast (Cairo) High School | 1:20.43 | Verified |
| 4 | CAHALAN, Lexi | 8 | Knox County High School | 1:20.08 | Verified |
| 5 | BARNETT, Reece | FR | Macon High School | 1:20.34 | Verified |
| 6 | AVERY, Phoenix | FR | South Shelby High School | 1:20.46 | Verified |
| 7 | WAGNER, Sharli | SO | Marion County High School | 1:28.85 | Verified |
| 8 | ARNOLD, Keyaira | FR | Atlanta High School | 1:51.38 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTIN, Mya | FR | Monroe City High School | 1:16.25 | Verified |
| 2 | MILLER, Jaelyn | FR | Westran High School | 1:12.87 | Verified |
| 4 | CLARK, Jaelynn | JR | Monroe City High School | 1:08.93 | Verified |
| 5 | KLEIN, Sydney | 8 | Silex High School | 1:09.89 | Verified |
| 6 | EWIGMAN, Shelby | SO | Marceline High School | 1:12.15 | Verified |
| 8 | PENNINGTON, Natalee | SO | Marceline High School | 1:19.90 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | YATES, KAYLIE | SO | Clark County High School | 1:07.13 | Verified |
| 2 | FISHER, Loralei | FR | Putnam County High School | 1:06.25 | Verified |
| 3 | BAILEY, Jersey | JR | Northeast (Cairo) High School | 1:04.17 | Verified |
| 4 | SHINN, Whitney | SR | North Shelby High School | 59.93 | Verified |
| 5 | PLENGE, Abby | SR | Clark County High School | 1:00.73 | Verified |
| 6 | WEIMER, Brooke | SR | Macon High School | 1:05.11 | Verified |
| 7 | ROUSH, Elizabelle | SO | South Shelby High School | 1:06.91 | Verified |
| 8 | COPENHAVER, Harper | FR | North Shelby High School | 1:08.78 | Verified |