| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | North Shelby High School | A | Fredrickson | Greening | Rich | Donath | 51.75 | Verified |
| 4 | Milan High School | A | Wilson | Alvarez | Ayala | Alvarez | 51.44 | Verified |
| 5 | Monroe City High School | A | Jones | McFall | Warden | Karr | 51.56 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marceline High School | A | Evans | Gooch | Cochran | Stallo | 48.94 | Verified |
| 2 | South Shelby High School | A | Moellering | Elsen | Wiseman | Hammond | 47.09 | Verified |
| 3 | Clark County High School | A | SHINN | WHEELER | HAMNER | Westercamp | 46.55 | Verified |
| 4 | Putnam County High School | A | Lopez | Tomlin | Ream | Perkins | 45.81 | Verified |
| 5 | Westran High School | A | Kribbs | Harlan | Stegall | Harlan | 46.50 | Verified |
| 6 | Missouri Military Academy | B | Kahle | Erskine | Quiroga | Salinas | NT | Verified |
| 7 | Macon High School | A | Carpenter | Harland | Carpenter | Reed | 47.28 | Verified |