| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Clark County High School | A | MCKAY | Lovell | NEVES | Veloso | NT | Unverified: |
| 5 | Marion County High School | A | Bock | Hathaway | Lee | Myers | 1:00.63 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Westran High School | A | Moran | Miller | Boyack | Hayes | 56.72 | Verified |
| 2 | Silex High School | A | Hall | Kuntz | Thoroughman | Weatherford | 55.01 | Verified |
| 3 | Northeast (Cairo) High School | A | Cross | Ellison | Hankins | Bailey | 53.91 | Verified |
| 4 | Putnam County High School | A | Seaton | Brundage | Knowles | Dickerson | 53.43 | Verified |
| 5 | Macon High School | A | Terry | McVicker | Anderson | Brown | 53.50 | Verified |
| 6 | South Shelby High School | A | Gaines | Eleazarraraz | Wilt | Roush | 54.34 | Verified |
| 7 | Marceline High School | A | Rodgers | Thornburg | Weese | Coe | 55.22 | Verified |
| 8 | Monroe City High School | A | Foss | Flick | Miller | Hays | 57.11 | Verified |