| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bishop DuBourg High School | A | Codada | Brickey | Reynolds | Spells | NT | Unverified: |
| 4 | Affton High School | A | Daughtry | Skinner | Smith | Manuel | NT | Unverified: |
| 5 | Lutheran South High School | A | Delaney | Maness | Labbee | Kasparek | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MICDS | A | Brothers | O'Basuyi | Jiang | Macam | NT | Unverified: |
| 2 | St. Louis Blue Knights | A | Nguyen | Plymale | Knoerr | Plymale | 2:14.12 | Verified |
| 3 | Chicago (St. Ignatius College Pr | A | 2:05.00 | Unverified: | ||||
| 4 | University City High School | A | Conners | Dixon | Bradley | Simon | 1:56.25 | Verified |
| 5 | Palos Heights (Shepard) | A | 1:56.50 | Unverified: | ||||
| 6 | Fox High School | A | Schepers | Rogers | Schwalbe | West | 2:08.92 | Verified |
| 7 | Principia High School | A | Dow | James | Hatwaizika | Oyugi | NT | Unverified: |
| 8 | Clayton High School | A | Miller | Sanders | Stern | Ramirez Taylor | NT | Unverified: |