| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BROWN, Sarah | SO | John Burroughs High School | NT | Unverified: |
| 4 | GARCIA-PINTOR, Joy | SO | Gateway Science Charter | NT | Unverified: |
| 5 | BOLIN, Moriah | SO | Lutheran South High School | NT | Unverified: |
| 6 | CELLA, Emelia | SO | Affton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NGUYEN, Sophia | SO | St. Louis Blue Knights | 1:06.17 | Verified |
| 2 | SHAFFER, Michelle | SO | Affton High School | 1:03.95 | Verified |
| 3 | WOLFE, Chloe | JR | Clayton High School | 1:01.13 | Verified |
| 4 | MAJORS, Emily | SO | St. Louis Blue Knights | 1:00.47 | Verified |
| 5 | TATKO, Anna | JR | Bishop DuBourg High School | 1:00.58 | Verified |
| 6 | HANSON, Sarah | JR | Lutheran South High School | 1:02.25 | Verified |
| 7 | DANIEL, Eliza | SO | Affton High School | 1:04.36 | Verified |
| 8 | LERENA, Lucia | SO | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAKER, Kaliyah | SO | Palos Heights (Shepard) | 56.35 | Verified |
| 2 | CROSS, Cesarine | SO | Clayton High School | 54.78 | Verified |
| 3 | DIXON, Laila | JR | University City High School | 52.18 | Verified |
| 4 | ANDERSON, Maya | SR | MICDS | 42.74 | Verified |
| 5 | MOTLEY, Kyla | SO | Palos Heights (Shepard) | 50.48 | Verified |
| 6 | PRANGE, Grace | FR | Lutheran South High School | 52.40 | Verified |
| 7 | GILL, Mia | SR | Palos Heights (Shepard) | 55.00 | Unverified: |
| 8 | GRESHAM, Hannah | JR | St. Louis Blue Knights | 58.96 | Verified |