| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | University City High School | A | Conners | Simon | Smith | Bradley | NT | Unverified: |
| 4 | Clayton High School | A | Stern | Sanders | Ramirez Taylor | Miller | NT | Unverified: |
| 5 | Affton High School | A | Cella | Karahasanovic | Lang | McFee | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MICDS | A | Brothers | Macam | O'Basuyi | Joe | NT | Unverified: |
| 2 | Bishop DuBourg High School | A | Codada | Brickey | Reynolds | Spells | 1:02.31 | Verified |
| 3 | Chicago (St. Ignatius College Pr | A | 58.00 | Unverified: | ||||
| 4 | Palos Heights (Shepard) | A | 53.10 | Unverified: | ||||
| 5 | Lutheran South High School | A | Dicken | Karsten | Labbee | Maness | 56.79 | Verified |
| 6 | Fox High School | A | Trompeter | Rogers | West | Wilson | 58.33 | Verified |
| 7 | St. Louis Blue Knights | A | Nguyen | Riggs | Knoerr | Plymale | 1:06.36 | Verified |