| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Collegiate School of Medicine an | A | Taylor | Bohlen | Robinson | Luster | NT | Unverified: |
| 3 | Gateway High School | A | Brown | Carothers | Bass | Nevils | 1:56.57 | Verified |
| 4 | STEAM Academy at McCluer South-B | A | Cromer | Saunders | Green | Bailey | 1:52.06 | Unverified: 11th Annual Bob G. Brunette Invi |
| 5 | Riverview Gardens High School | A | Coleman | Green | Robinson | Lewis | 1:55.50 | Verified |
| 6 | Miller Career Academy | A | Kershaw | Moore | Powell | Washington | 2:14.41 | Verified |