| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | KIPP St. Louis | A | Baker | Burch | Moore | Cook | 3:53.25 | Verified |
| 4 | Riverview Gardens High School | A | Caph | Walker | Williams | McGhee | 3:40.00 | Unverified: |
| 5 | STEAM Academy at McCluer South-B | A | Moreland-Bell | Coleman | Ivy | Fields | 3:40.56 | Unverified: |
| 6 | Gateway High School | A | Spencer | Warren | Spencer | Zevulun | 4:25.29 | Verified |