| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Gateway High School | A | Carothers | Brown | Bass | Ingabirse | NT | Unverified: |
| 4 | Riverview Gardens High School | A | Coleman | Jackson | Washington | Holland | 4:21.52 | Verified |
| 5 | STEAM Academy at McCluer South-B | A | Green | Cromer | Gibson | Young | 4:27.89 | Unverified: Hazelwood Invitational |
| 6 | Miller Career Academy | A | NT | Unverified: |