| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ROUNDTREE, Maliaya | JR | Roosevelt High School | NT | Unverified: |
| 3 | WALKER, Aniya | SO | Riverview Gardens High School | NT | Unverified: |
| 4 | DAVIS, Tsoti Khymor | SO | KIPP St. Louis | 32.65 | Verified |
| 5 | GLOVER, Samiyah | SO | Roosevelt High School | 35.64 | Verified |
| 6 | NEVILS, Kennadi | JR | Gateway High School | NT | Unverified: |
| 7 | HARRIS-GRAYER, Amyah | FR | Riverview Gardens High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KERSHAW, Janiyah | FR | Miller Career Academy | 32.15 | Verified |
| 2 | RUFUS-SMITH, Savannah | JR | STEAM Academy at McCluer South-B | 32.03 | Verified |
| 3 | ROBINSON, Markayla | JR | Collegiate School of Medicine an | 29.87 | Verified |
| 4 | CAROTHERS, Kennedy | JR | Gateway High School | 29.51 | Verified |
| 5 | GREEN, Aniyah | SO | Riverview Gardens High School | 29.80 | Verified |
| 6 | PRIMUS, Daphne | FR | Collegiate School of Medicine an | 30.32 | Verified |
| 7 | MAYHEW, Arryona | SO | Roosevelt High School | 32.11 | Verified |
| 8 | BROWN, Brieanna | SO | KIPP St. Louis | 32.50 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MIMES, Angel | SO | Miller Career Academy | 28.70 | Verified |
| 2 | FLECHER, Jasmine | JR | Miller Career Academy | 28.28 | Verified |
| 3 | BAILEY, Riyan | FR | STEAM Academy at McCluer South-B | 27.08 | Unverified: |
| 4 | VANDENBRINK, Alia | JR | Collegiate School of Medicine an | 25.29 | Verified |
| 5 | HUBBARD, Demea | FR | STEAM Academy at McCluer South-B | 26.79 | Unverified: |
| 6 | BROWN, Tiara | JR | Gateway High School | 27.36 | Verified |
| 7 | GREEN, Darriana | SO | KIPP St. Louis | 28.52 | Verified |
| 8 | WOODS, Na'Tavia | FR | McKinley Classical Leadership Hi | 29.42 | Verified |