| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HERNDON, Divine | SO | Riverview Gardens High School | 1:01.37 | Verified |
| 3 | RICHARDSON, Mariah | FR | STEAM Academy at McCluer South-B | 58.38 | Verified |
| 4 | SAUNDERS, Sa'Niya | JR | STEAM Academy at McCluer South-B | 48.96 | Verified |
| 5 | GLADDEN, Mykale | SR | Riverview Gardens High School | 52.94 | Verified |
| 6 | MCFOWLAND, Pamela | SO | Riverview Gardens High School | 58.53 | Verified |