| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CLARK, Raiveon | SR | Roosevelt High School | NT | Unverified: |
| 3 | JAMES, Lee | FR | Miller Career Academy | NT | Unverified: |
| 4 | BRAND, Gabriel | FR | Miller Career Academy | NT | Unverified: |
| 5 | HAMPTON, Treshawn | SO | Collegiate School of Medicine an | NT | Unverified: |
| 6 | WILLIAMS, Serjei | SR | Riverview Gardens High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POWELL, Mario | FR | Riverview Gardens High School | NT | Unverified: |
| 2 | KEMP, Xavier | JR | KIPP St. Louis | 1:04.41 | Verified |
| 3 | JOHNSON, Amari | SO | Riverview Gardens High School | 1:03.03 | Verified |
| 4 | FULTON, Brock | SO | McKinley Classical Leadership Hi | 1:02.09 | Verified |
| 5 | MILTON, Kevion | SO | Roosevelt High School | 1:02.66 | Verified |
| 6 | UPCHURCH, Darrion | FR | McKinley Classical Leadership Hi | 1:03.33 | Verified |
| 7 | PRUITT, Darren | FR | McKinley Classical Leadership Hi | 1:05.19 | Verified |
| 8 | CLAY, Dante | FR | Gateway High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, Anthony | JR | Collegiate School of Medicine an | 1:00.99 | Verified |
| 2 | BARNETT, Ne'Miah | JR | STEAM Academy at McCluer South-B | 58.45 | Unverified: |
| 3 | FIELDS, Leonard | JR | STEAM Academy at McCluer South-B | 56.99 | Verified |
| 4 | COOK, Trey | SR | KIPP St. Louis | 51.67 | Verified |
| 5 | IVY, Joziah | SR | STEAM Academy at McCluer South-B | 55.01 | Unverified: |
| 6 | RAY, Rylan | JR | KIPP St. Louis | 58.36 | Verified |
| 7 | SPENCER, Laron | FR | Gateway High School | 1:00.26 | Verified |
| 8 | BLAND, Eric | JR | Gateway High School | 1:01.44 | Verified |