| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Collegiate School of Medicine an | A | Taylor | Bohlen | Vaughn | Luster | NT | Unverified: |
| 3 | Riverview Gardens High School | A | Ray | Coleman | Davis | Lewis | 55.90 | Unverified: |
| 4 | STEAM Academy at McCluer South-B | A | Hubbard | Cromer | Clark | Jones | 51.58 | Unverified: 11th Annual Bob G. Brunette Invi |
| 5 | Gateway High School | A | Brown | Carothers | Nevils | N'Guessan | 54.47 | Verified |
| 6 | Miller Career Academy | A | Kershaw | Flecher | Orr | Mimes | 1:00.62 | Verified |
| 7 | McKinley Classical Leadership Hi | A | Noel | Stuckey | Woods | Powe | NT | Unverified: |