| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Roxana | A | Caruthers | Schmidt | Hardin | McBride | 1:15.00 | |
| 4 | Bethalto (Civic Memorial) | A | Klaas | Books | Davenport | Piening | 1:00.00 | |
| 6 | Jerseyville (Jersey) | A | Schultz | Burch | Schleeper | Kribs | 1:13.00 | |
| 8 | Highland | A | Irving | Schwarz | Diller | Crowe | NT |