| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Highland | A | Smith | Mancinas | Grotefendt | Basden | 11:00.00 | |
| 2 | Jerseyville (Jersey) | A | Turnbaugh | Edwards | Little | Wade | 11:00.00 | |
| 3 | Bethalto (Civic Memorial) | A | Collman | Zampieri | Naugle | Dutton | 11:20.00 | |
| 4 | Wood River (East Alton-W.R.) | A | Baker | Gould | Simpson-Kolmer | Loeffelman | 12:20.00 | |
| 5 | Roxana | A | Doyle | Newton | Ponce | Hardin | 12:29.24 |