| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 6 | Bethalto (Civic Memorial) | A | Meiser | Brousseau | Hannaford | Mann | 13:45.00 | |
| 7 | Alton (Marquette) | A | Rister | Wells | Certa | Johnson | NT | |
| 8 | Mascoutah | A | Hart | Plotts | Haas | Dimmitt | 17:05.65 | |
| 9 | Roxana | A | Losch | Arview | Partridge | Doyle | NT | |
| 10 | Jerseyville (Jersey) | A | Lorton | Soffray | Finkes | Steinacher | NT | |
| 11 | Highland | A | Perkins | Pace | Marron | Dortch | NT |