| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mascoutah | A | Carter | Vaquerano | Brooks | Beech | 1:30.00 | |
| 4 | Bethalto (Civic Memorial) | A | Anderson | Coffman | Crockett | Bellanca | 1:05.00 | |
| 6 | Jerseyville (Jersey) | A | Yates | Trexler | Shaffer | Weber | 1:17.00 |