| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Highland | A | Choat | Thomas | Maas | Frey | NT | |
| 3 | Bethalto (Civic Memorial) | A | Crymes | Brousseau | Govero | Vaughn | 2:15.00 | |
| 4 | Mascoutah | A | Carter | Haas | Dimmitt | Doyle | 2:24.73 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Alton (Marquette) | A | Cain | Davenport | Hentrich | Wells | 2:04.00 | |
| 3 | Wood River (East Alton-W.R.) | A | Minner | Sanders | Brown | Truax | 2:01.10 | |
| 4 | Jerseyville (Jersey) | A | Yamnitz | Leslie | Peterbaugh | Allen | 2:03.00 | |
| 5 | Roxana | A | Lohman | Carlisle | Daugherty | Eads | 2:10.00 |