| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bethalto (Civic Memorial) | A | Silva | Wooten | Piening | Fonrodona | 46.80 | |
| 2 | Highland | A | Graham | Story | Nimmo | Wallace | 45.45 | |
| 3 | Roxana | A | Coles | Wieneke | Osmoe | McBride | 44.02 | |
| 4 | Jerseyville (Jersey) | A | Withrow | Talley | Borkowski | Jones | 44.70 | |
| 5 | Wood River (East Alton-W.R.) | A | Skinner | Marshall | Burgess Jr | Burch | 46.50 |