| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Alton (Marquette) | A | Downey | Stephenson | Bryson | Rae | 58:00.00 | |
| 3 | Jerseyville (Jersey) | A | Yamnitz | Deist | Leslie | Trexler | 56.71 | |
| 4 | Bethalto (Civic Memorial) | A | Crymes | Shields | Govero | Brousseau | 58.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Highland | A | Henschen | Pollard | Thomas | Maas | 54.68 | |
| 3 | Mascoutah | A | Thompkins | Caskey | Maydwell | Williams | 53.43 | |
| 4 | Roxana | A | Lohman | Carlisle | Lohman | Daugherty | 53.99 | |
| 5 | Wood River (East Alton-W.R.) | A | Brown | Minner | Sanders | Truax | 55.10 |