| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wood River (East Alton-W.R.) | A | Skinner | Marshall | Burgess Jr | Lane | NT | |
| 2 | Roxana | A | Schmidt | Meadows | McBride | Doyle | 3:48.02 | |
| 3 | Highland | A | Fridley | Basden | Mancinas | Miller | 3:40.82 | |
| 4 | Alton (Marquette) | A | Fears | Kline | Nash | Rochester | 3:42.00 | |
| 5 | Bethalto (Civic Memorial) | A | Flanagan | Zampieri | Kearbey | Eldridge | 3:50.00 | |
| 6 | Jerseyville (Jersey) | A | Burch | Edwards | Deist | Wilson | NT |