| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bethalto (Civic Memorial) | A | Wehmeier | Crockett | Anderson | Coffman | 5:05.00 | |
| 2 | Jerseyville (Jersey) | A | Allen | Brooks | Peterbaugh | Weber | 4:50.83 | |
| 3 | Alton (Marquette) | A | Johnson | Wells | Davenport | Certa | 4:38.00 | |
| 4 | Mascoutah | A | Uptergrove | Mak | Haas | Dimmitt | 4:40.22 | |
| 5 | Highland | A | Keeney | Friederich | Gurley | Peters | 4:55.10 |