| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bethalto (Civic Memorial) | A | Naugle | Dutton | Cranford | Collman | 8:30.00 | |
| 2 | Highland | A | Smart | Rednour | Roach | Steinbeck | 8:45.67 | |
| 3 | Roxana | A | Doyle | Ponce | Blas | Gilbert | 9:14.10 | |
| 4 | Wood River (East Alton-W.R.) | A | Baker | Simpson-Kolmer | Gould | Loeffelman | NT | |
| 5 | Jerseyville (Jersey) | A | Little | Turnbaugh | Bishop | McCoy | 10:00.00 |