| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Alton (Marquette) | A | Fears | Kline | Nash | Rochester | 1:42.00 | |
| 2 | Highland | A | Fridley | Nimmo | Walker | Schwarz | 1:37.64 | |
| 3 | Jerseyville (Jersey) | A | Withrow | Talley | Jones | Borkowski | 1:35.00 | |
| 4 | Roxana | A | Ellis | Wieneke | McBride | Osmoe | DQ | |
| 5 | Wood River (East Alton-W.R.) | A | Skinner | Marshall | Burgess Jr | Burch | 1:38.30 | |
| 6 | Bethalto (Civic Memorial) | A | Silva | Turner | Wooten | Atteberry | 1:42.00 |