| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bethalto (Civic Memorial) | A | Crymes | Wehmeier | Govero | Shields | 2:03.00 | |
| 3 | Roxana | A | Carlisle | Springman | Eads | Lohman | 1:59.70 | |
| 4 | Alton (Marquette) | A | Cain | Bryson | Downey | Rae | 2:00.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mascoutah | A | Hubard | Thompkins | Carter | Maydwell | 1:57.70 | |
| 3 | Wood River (East Alton-W.R.) | A | Brown | Minner | Sanders | Truax | 1:57.10 | |
| 4 | Highland | A | Henschen | Pollard | Maas | Thomas | 1:57.31 | |
| 5 | Jerseyville (Jersey) | A | Deist | Leslie | Weber | Yates | 1:58.75 |