| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bethalto (Civic Memorial) | A | Hannaford | Mann | Bellanca | Meiser | 10:37.00 | |
| 2 | Mascoutah | A | Doyle | Perryman | Akins | Hart | 11:24.94 | |
| 3 | Highland | A | Marron | Dortch | Frey | Pace | 11:35.03 | |
| 4 | Alton (Marquette) | A | Johnson | Rister | Eccles | Certa | 11:50.00 | |
| 5 | Roxana | A | Doyle | Curren | Partridge | Arview | 12:11.00 | |
| 6 | Jerseyville (Jersey) | A | Brooks | Lorton | Soffray | Finkes | 12:28.94 |