| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ritenour High School | A | Phillips | Henderson | Monroe | Shaw | NT | Unverified: |
| 2 | STEAM Academy at McCluer South-B | A | Hubbard | Young | Bailey | Richardson | 55.83 | Unverified: |
| 3 | East St. Louis (Sr.) | A | 52.87 | Unverified: | ||||
| 4 | Belleville (East) | A | London | Darielle | Ashayla | KaLiyah | .50 | Unverified: |
| 5 | McCluer North High School | A | Davis | Moore | Clayton | Hart | 51.65 | Verified |
| 6 | Hazelwood Central High School | A | Wicks | Scruggs | Sheppard | Griffin | 54.30 | Unverified: |
| 7 | Vashon High School | A | Simpson-Bey | Evans | Jones | Smith | NT | Verified |
| 8 | Riverview Gardens High School | A | Davis | Coleman | Harris-Grayer | Lewis | NT | Unverified: |