| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Hazelwood West High School | A | NT | Unverified: | ||||
| 4 | McCluer High School | A | Walker | Williams | III | Reynolds | NT | Unverified: |
| 5 | University City High School | A | Plummer | Holland | Robinson | Jennings | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vashon High School | A | Brown | Cody | Taylor | Aaron | 1:45:03.00 | Unverified: |
| 2 | STEAM Academy at McCluer South-B | A | Simpson-Johnson | Boyd | Frenchie | Holliman | 1:38.99 | Unverified: |
| 3 | Cardinal Ritter High School | A | 1:36.03 | Unverified: | ||||
| 4 | Riverview Gardens High School | A | 1:31.64 | Verified | ||||
| 5 | Hazelwood Central High School | A | Williams | Martin | Thornton | Anthony | 1:33.57 | Unverified: |
| 6 | East St. Louis (Sr.) | A | Baker | Smith | McClain | Mosley | 1:38.50 | Unverified: |
| 7 | Belleville (East) | A | NT | Unverified: | ||||
| 8 | Lift for Life Academy Charter Hi | A | Jones | Hightower | Young | Lumpkin | NT | Unverified: |