| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAEHR, Adam | SO | Rock Bridge High School | 2:11.01 | Verified |
| 2 | CHINIKOSKI, Henrique | SO | Rock Bridge High School | 2:13.71 | Verified |
| 3 | MCGOWAN, Kyle | SO | Christian Fellowship School | 2:23.90 | Verified |
| 4 | GAINES, Charles | SO | Battle High School | 2:26.92 | Verified |
| 5 | WEBSTER, Talib | JR | Battle High School | 2:38.04 | Verified |
| 6 | JOHNSON, Terrel | JR | Battle High School | 2:39.70 | Verified |
| 7 | KAUFFMAN, Nathaniel | SO | Christian Fellowship School | 2:41.41 | Verified |
| 8 | STEPNEY-NEAL, Jalen | SR | Battle High School | 2:42.20 | Verified |
| 9 | CURRENT, Nick | FR | Hickman High School | NT | Unverified: |
| 10 | VILLASANA, Reed | SO | Hickman High School | NT | Unverified: |
| 11 | MUGISHA, Habimana | SR | Battle High School | NT | Unverified: |
| 12 | SUMMERALL, William | FR | Rock Bridge High School | NT | Unverified: |
| 13 | CLAPP, Isaiah | FR | Battle High School | NT | Unverified: |
| 14 | BRADSHAW, Ahmadrion | FR | Battle High School | NT | Unverified: |
| 15 | BRADSHAW, Adrion | FR | Battle High School | NT | Unverified: |
| 16 | WISE, Colin | FR | Hickman High School | NT | Unverified: |