| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | EASTMAN, Ellie | SO | Hickman High School | NT | Unverified: |
| 3 | WEBER, Iris | FR | Hickman High School | NT | Unverified: |
| 4 | IRELAND, Isabelle | FR | Rock Bridge High School | NT | Unverified: |
| 5 | MAHANEY, Amanda | JR | Battle High School | NT | Unverified: |
| 6 | BRIDGEFORD, Isabella | SO | Battle High School | NT | Unverified: |
| 7 | MCNALLY, Nora | SO | Hickman High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WHITFIELD, Jasmine | SO | Battle High School | NT | Unverified: |
| 2 | SALL, Elizabeth | FR | Hickman High School | 1:18.25 | Verified |
| 3 | MOORE, Bailey | FR | Hickman High School | 1:06.89 | Verified |
| 4 | HESTON, Grace | FR | Christian Fellowship School | 1:02.71 | Verified |
| 5 | MILLER, Lauren | SO | Rock Bridge High School | 1:02.91 | Verified |
| 6 | DOMEIER, Isabelle | SO | Hickman High School | 1:07.06 | Verified |
| 7 | PATRICK, Saryia | JR | Battle High School | NT | Unverified: |
| 8 | O'LAUGHLIN, Zoe | FR | Hickman High School | NT | Unverified: |