| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BOSTICK, Jozelynn | SR | Centralia High School | 5:23.71 | Verified |
| 3 | DORSEY, Jocelyn | JR | Hannibal High School | 5:36.95 | Verified |
| 4 | MOSS, Kenedee | FR | Centralia High School | 5:38.07 | Verified |
| 5 | ELLIS, Matera | SR | Mark Twain High School | 6:01.00 | Verified |
| 6 | HAYS, Ella | JR | Monroe City High School | 6:10.65 | Verified |
| 7 | BONVILLIAN, Ava | SO | Hannibal High School | 6:13.85 | Verified |
| 8 | STAHL, Kaycie | JR | Highland High School | 6:20.24 | Verified |
| 9 | JOHNSON, Avery | SO | Monroe City High School | 6:23.39 | Verified |
| 10 | SUBLETTE, Mallory | SR | Palmyra High School | 6:31.24 | Verified |
| 11 | BURKS, Stella | JR | Macon High School | 6:33.90 | Verified |
| 12 | JOHNSON, Mia | JR | Clark County High School | 6:34.65 | Verified |
| 13 | LITTLE, TRINITY | SO | Clark County High School | 6:38.35 | Verified |
| 14 | SEILER, Elizabeth | JR | Macon High School | 6:41.03 | Verified |
| 15 | WEIMAN, Ava | JR | Palmyra High School | 6:54.13 | Verified |
| 16 | STUPAVSKY, Isabella | SR | Monroe City High School | 6:58.52 | Verified |
| 17 | RUFENER, Tadara | SR | South Shelby High School | 7:01.26 | Verified |
| 18 | BATES, Emilia | SO | Hannibal High School | 7:05.60 | Verified |
| 19 | HAYS, Marissa | FR | Monroe City High School | 7:46.31 | Verified |
| 20 | PUTERBAUGH, Jordan | FR | Hannibal High School | NT | Unverified: |
| 21 | SATTERFIELD, Zafaran | FR | Southern Boone County High Schoo | NT | Unverified: |