| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clark County High School | A | Billings | Westercamp | DAVIDSON | WHEELER | NT | Unverified: |
| 3 | South Shelby High School | B | Moellering | Elsen | Bowen | Hetheriton | 1:43.00 | Unverified: |
| 4 | Macon High School | A | Carpenter | Kiser | Reed | Martie | 1:41.02 | Verified |
| 5 | Palmyra High School | A | Bier | Harper | Hicks | Redd | 1:42.91 | Verified |
| 6 | Monroe City High School | A | Lehenbauer | McFall | Warden | Shuck | 1:56.88 | Verified |
| 7 | Highland High School | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Southern Boone County High Schoo | A | Trittler | Amelunke | Jackson | Talley | NT | Verified |
| 2 | Louisiana High School | A | Greene | Dzierwa | Luck | Gamble | 1:39.37 | Verified |
| 3 | Centralia High School | A | Beard | Rosenfelder | Whisler | Dunn | 1:38.85 | Verified |
| 4 | Hannibal High School | A | Essig | Turner | Bergheger | Sharkey | 1:34.19 | Verified |
| 5 | South Shelby High School | A | Moellering | Wilt | Wiseman | Hammond | 1:37.09 | Verified |
| 6 | Mark Twain High School | A | Bergthold | Tobin | Bennett | Zumwalt | 1:39.09 | Verified |
| 7 | Southern Boone County High Schoo | B | Straatmann | Lutz | Talley | Shelton | 1:39.87 | Verified |
| 8 | Hannibal High School | B | Odom | Bailey | Ferreira | Hall | DQ | Unverified: |