| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mark Twain High School | A | Boleach | Hudelson | Hawn | Dotson | NT | Unverified: |
| 3 | Monroe City High School | B | Foss | Flick | Miller | Pfaff | 2:05.82 | Verified |
| 4 | Hannibal High School | B | Lightle | Sherman | Vandiver | Williams | 2:02.00 | Unverified: |
| 5 | Monroe City High School | A | Clark | Lehenbauer | Martin | Hays | 2:05.82 | Verified |
| 6 | Clark County High School | A | Lovell | IRVIN | West | Veloso | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Louisiana High School | A | Campbell | Ries | Pedersen | McGlothin | 1:56.86 | Verified |
| 2 | Macon High School | A | Terry | McVicker | Anderson | Weimer | 1:56.13 | Verified |
| 3 | Palmyra High School | A | Redd | Loman | Compton | White | 1:54.86 | Verified |
| 4 | Centralia High School | A | Sontheimer | Hawkins | Walters | Lewis | 1:49.66 | Verified |
| 5 | Hannibal High School | A | Brown | Dean | LaJoy | Stewart | 1:53.25 | Verified |
| 6 | Highland High School | A | Sharpe | Keith | Bringer | Reed | 1:55.72 | Verified |
| 7 | South Shelby High School | A | McWilliams | Mitchell | Wilt | Roush | 1:56.19 | Verified |
| 8 | Southern Boone County High Schoo | A | Germann | Patton | Hinton | Johnson | 1:58.89 | Verified |