| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HOLLENDER, Brinley | JR | Clark County High School | 22.09 | Verified |
| 3 | LEWIS, Bianca | SO | Macon High School | 21.36 | Verified |
| 5 | HICKS, Sophie | FR | Mark Twain High School | 20.75 | Verified |
| 6 | KAUFFMAN, Emily | FR | Macon High School | 21.68 | Verified |
| 7 | RILEY, Chloe | FR | Hannibal High School | 22.47 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NORVELL, Harley | JR | Louisiana High School | 20.28 | Verified |
| 2 | HATHAWAY, Kennedy | FR | Marion County High School | 19.33 | Verified |
| 4 | SONTHEIMER, Harper | SR | Centralia High School | 18.46 | Unverified: |
| 5 | FOSS, Gracee | SO | Monroe City High School | 18.87 | Verified |
| 6 | BERGTHOLD, Jordan | SR | Southern Boone County High Schoo | 19.24 | Verified |
| 7 | FANNING, Addison | FR | Southern Boone County High Schoo | 19.79 | Verified |
| 8 | MCDOWELL, Ericka | FR | Clark County High School | 20.57 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MITCHELL, Kamryn | JR | South Shelby High School | 18.07 | Verified |
| 2 | MCGLOTHIN, De'Aira | SO | Louisiana High School | 17.76 | Verified |
| 3 | HARK, Nora | SR | Hannibal High School | 17.13 | Verified |
| 4 | COMPTON, Sydney | SO | Palmyra High School | 15.69 | Verified |
| 5 | VOSS, Tenlee | SR | Palmyra High School | 17.12 | Verified |
| 6 | BRINGER, Ansley | SR | Highland High School | 17.72 | Verified |
| 7 | YOUNGBLOOD, Audri | SO | Monroe City High School | 18.02 | Verified |
| 8 | BROWN, Riyenn | SO | Hannibal High School | 18.25 | Verified |