| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LITTLE, Tianna | FR | Clark County High School | NM | Unverified: |
| 2 | FLICK, Rylee | FR | Monroe City High School | NM | Unverified: |
| 3 | HESSELL, Makenzie | FR | Macon High School | NM | Unverified: |
| 4 | LAWSON, Jade | JR | Centralia High School | 1.22m | Verified |
| 5 | FOX, Matilda | SO | Centralia High School | 1.25m | Verified |
| 6 | KAUFFMAN, Emily | FR | Macon High School | 1.27m | Verified |
| 7 | VANDIVER, Sophia | SO | Hannibal High School | 1.28m | Verified |
| 8 | CONIGLIO, Sydney | FR | Hannibal High School | 1.29m | Verified |
| 9 | STUPAVSKY, Isabella | SR | Monroe City High School | 1.32m | Verified |
| 10 | TRIPLETT, Janie | FR | Palmyra High School | 1.35m | Verified |
| 11 | HAWN, Kadence | SR | Mark Twain High School | 1.37m | Verified |
| 12 | AMELUNKE, Kate | JR | Southern Boone County High Schoo | 1.40m | Verified |
| 13 | WILT, Madison | SO | South Shelby High School | 1.40m | Verified |
| 14 | MILLER, Payton | SR | Highland High School | 1.40m | Verified |
| 15 | EVANS, Mya | FR | Hannibal High School | 1.42m | Verified |
| 16 | NELSON, Amarra | JR | South Shelby High School | 1.44m | Verified |
| 17 | MAYES, LilliAnn | SO | Macon High School | 1.47m | Verified |
| 18 | BOWSER, Raegan | JR | Louisiana High School | 1.49m | Verified |
| 19 | BUKOWSKY, Chloe | JR | Southern Boone County High Schoo | 1.52m | Verified |
| 20 | HICKS, Chloe | JR | Palmyra High School | 1.54m | Verified |
| 21 | PEDERSEN, Jordan | SO | Louisiana High School | 1.56m | Verified |
| 22 | NOVEL, Taegen | SO | Hannibal High School | 1.59m | Verified |