| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Palmyra High School | A | Harper | Redd | Sheppard | Reid | NT | Unverified: |
| 4 | Hannibal High School | B | Bailey | Worthington | Odom | Davis | 4:00.00 | Unverified: |
| 5 | Southern Boone County High Schoo | A | Rehg | Ottinger | Marc | Trittler | 4:01.05 | Verified |
| 6 | Highland High School | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marion County High School | A | Martin | Tuley | Wood | Stotts | 3:59.74 | Verified |
| 2 | Monroe City High School | A | Karr | Wheeler | Lehenbauer | Woolen | 3:49.81 | Verified |
| 3 | Hannibal High School | A | Nichols | Essig | Pociask | Hess | 3:47.78 | Verified |
| 4 | South Shelby High School | A | Hammond | Elsen | Wiseman | Wilt | 3:39.73 | Verified |
| 5 | Mark Twain High School | A | Preston | Evans | Miller | Northcutt | 3:44.59 | Verified |
| 6 | Centralia High School | A | Whitlatch | Mitchell | Holiman | Dunn | 3:49.19 | Verified |
| 7 | Clark County High School | A | SHINN | Day | Westercamp | WHEELER | 3:55.74 | Verified |