| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Southern Boone County High Schoo | A | Volkart | Satterfield | Patton | Germann | NT | Unverified: |
| 4 | Mark Twain High School | A | Hudelson | Norton | Dotson | Miller | 4:54.21 | Verified |
| 5 | Louisiana High School | A | Lesley | Sperry | Bowser | Lentz | 5:07.69 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal High School | B | Coniglio | Johnson | Riley | Sherman | NT | Unverified: |
| 2 | Highland High School | A | Reed | Keith | Abell | Sharpe | 4:42.60 | Verified |
| 3 | Palmyra High School | A | Sheputis | Leon | Loman | White | 4:33.15 | Verified |
| 4 | Centralia High School | A | Adams | Erisman | Sontheimer | Lewis | 4:20.97 | Verified |
| 5 | South Shelby High School | A | Mitchell | Roush | Eleazarraraz | McWilliams | 4:27.39 | Verified |
| 6 | Hannibal High School | A | Ball | Groth | Karr | Waelder | 4:34.32 | Verified |
| 7 | Clark County High School | A | Plenge | Little | WEAVER | YATES | 4:44.01 | Unverified: Davis County Boarder Battle |