| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal High School | A | Hess | Thompson | Munger | Nichols | 8:50.91 | Verified |
| 2 | Centralia High School | A | Moss | Bostick | Holiman | Lesher | 8:53.58 | Verified |
| 3 | Monroe City High School | A | Lehenbauer | Richardson | Lorenson | Hunter | DQ | Verified |
| 4 | Southern Boone County High Schoo | A | Cabrera | Kitch | Ottinger | Eppy | 9:22.53 | Verified |
| 5 | Marion County High School | A | Britt | Schluckebier | Wood | Wright | 9:28.57 | Verified |
| 6 | Clark County High School | A | Hurt | Wardlow | McDaniel | Day | 9:38.82 | Verified |
| 7 | Palmyra High School | A | Bross | Bier | MASON KIEFABER | Jurado | 9:48.23 | Verified |
| 8 | Highland High School | A | NT |