| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | LIZARRAGA-ZUBIETA, Danna | SR | Southern Boone County High Schoo | NT | Unverified: |
| 4 | MILLER, Makenna | FR | Monroe City High School | NT | Unverified: |
| 5 | HATHAWAY, Kennedy | FR | Marion County High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WALLACE, Katie | SO | Louisiana High School | 34.36 | Verified |
| 2 | IRVIN, ISABELLA | SO | Clark County High School | 33.01 | Verified |
| 3 | MARTIN, Mya | FR | Monroe City High School | 32.42 | Verified |
| 4 | BAKER, Chezney | FR | Centralia High School | 31.74 | Verified |
| 5 | PATTON, Jewels | SO | Southern Boone County High Schoo | 32.40 | Verified |
| 6 | CRIST, Bailie | FR | Highland High School | 32.76 | Verified |
| 7 | SLATER, Sophia | FR | Canton High School | 33.66 | Verified |
| 8 | WAGNER, Sharli | SO | Marion County High School | 37.03 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JOHNSON, Nevaeh | SO | Hannibal High School | 31.20 | Verified |
| 3 | ROSS, Audrey | SR | Mark Twain High School | 31.13 | Verified |
| 4 | GAINES, Hannah | FR | South Shelby High School | 30.41 | Verified |
| 5 | CLARK, Jaelynn | JR | Monroe City High School | 30.47 | Verified |
| 6 | BOCK, Maya | FR | Marion County High School | 31.16 | Verified |
| 7 | LESLEY, Michela | FR | Louisiana High School | 31.31 | Verified |
| 8 | INCE, Lauren | SO | Louisiana High School | 31.65 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOX, Matilda | SO | Centralia High School | 30.21 | Verified |
| 2 | BARNETT, Reece | FR | Macon High School | 29.73 | Verified |
| 3 | YATES, KAYLIE | SO | Clark County High School | 29.46 | Verified |
| 4 | LOMAN, Alaina | JR | Palmyra High School | 29.06 | Unverified: |
| 5 | HAWN, Kadence | SR | Mark Twain High School | 29.40 | Verified |
| 6 | CRANE, Kameil | JR | Hannibal High School | 29.57 | Verified |
| 7 | TASCO, Amelia | SR | Highland High School | 29.92 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCBRIDE, Bella | JR | Palmyra High School | 27.96 | Verified |
| 2 | DEAN, Sameya | SO | Hannibal High School | 27.70 | Verified |
| 3 | WHITE, Taytum | JR | Palmyra High School | 27.12 | Verified |
| 4 | LEWIS, Shelby | JR | Centralia High School | 26.30 | Verified |
| 5 | ANDERSON, Brooklyn | SR | Macon High School | 26.88 | Verified |
| 6 | PLENGE, Abby | SR | Clark County High School | 27.34 | Verified |
| 7 | TERRY, Ella | SO | Macon High School | 27.84 | Verified |
| 8 | BOLEACH, Madison | JR | Mark Twain High School | 28.17 | Verified |