| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | MCDOWELL, Ericka | FR | Clark County High School | NT | Unverified: |
| 4 | RILEY, Chloe | FR | Hannibal High School | NT | Unverified: |
| 5 | HICKS, Sophie | FR | Mark Twain High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HOLLENDER, Brinley | JR | Clark County High School | 1:01.98 | Verified |
| 4 | FOSS, Gracee | SO | Monroe City High School | 54.39 | Verified |
| 5 | NEVES, LUNDYN | JR | Clark County High School | 57.62 | Verified |
| 6 | BERGTHOLD, Jordan | SR | Southern Boone County High Schoo | 1:02.12 | Verified |
| 7 | AMELUNKE, Kate | JR | Southern Boone County High Schoo | 1:05.25 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCVICKER, Danni | SO | Macon High School | 53.55 | Verified |
| 2 | ERISMAN, Rebecca | FR | Centralia High School | 50.80 | Verified |
| 3 | SONTHEIMER, Harper | SR | Centralia High School | 50.20 | Verified |
| 4 | YOUNGBLOOD, Audri | SO | Monroe City High School | 48.26 | Verified |
| 5 | VOSS, Tenlee | SR | Palmyra High School | 49.96 | Verified |
| 6 | BRINGER, Ansley | SR | Highland High School | 50.78 | Verified |
| 7 | HARK, Nora | SR | Hannibal High School | 51.07 | Verified |
| 8 | BROWN, Riyenn | SO | Hannibal High School | 54.21 | Verified |