| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Monroe City High School | A | Jones | Karr | Warden | McFall | 51.56 | Verified |
| 3 | Palmyra High School | A | Bier | Hicks | Pennewell | Redd | 49.66 | Verified |
| 4 | Mark Twain High School | A | Bergthold | Zumwalt | Bennett | Tobin | 48.09 | Verified |
| 5 | Centralia High School | A | Baker | Beard | Rosenfelder | Whisler | 48.54 | Verified |
| 6 | South Shelby High School | B | Bowen | Hetheriton | Moellering | Ratliff | 51.00 | Unverified: |
| 7 | Highland High School | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Southern Boone County High Schoo | A | Amelunke | Jennings | Marc | Jackson | 47.18 | Verified |
| 2 | South Shelby High School | A | Moellering | Wilt | Wiseman | Hammond | 47.09 | Verified |
| 3 | Clark County High School | A | SHINN | DAVIDSON | WHEELER | HAMNER | 46.55 | Verified |
| 4 | Hannibal High School | A | Essig | Whiteside | Bergheger | Turner | 44.22 | Verified |
| 5 | Louisiana High School | A | Greene | Dzierwa | Luck | Blackwell | 46.30 | Verified |
| 6 | Hannibal High School | B | Bailey | Hall | Odom | Ferreira | 47.00 | Unverified: |
| 7 | Southern Boone County High Schoo | B | Hunolt | Talley | Bishop | Shelton | NT | Verified |
| 8 | Macon High School | A | Carpenter | Harland | Carpenter | Reed | 47.56 | Verified |