| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARTMAN, Lauren | SR | Crystal City High School | 5:43.48 | Verified |
| 2 | SANSEGRAW, Celeste | SR | Potosi High School | 5:47.05 | Verified |
| 3 | TERRELL, Katelyn | SO | Grandview-R2 (Hillsboro) High Sc | 5:56.05 | Verified |
| 4 | DRYER, Grace | FR | Pacific High School | 6:09.32 | Verified |
| 5 | WENGER, Madeline | SO | Rosati-Kain High School | 6:16.41 | Verified |
| 6 | LAND, Allison | SO | Potosi High School | 6:20.75 | Verified |
| 7 | VOGELSANG, Natalee | JR | Grandview-R2 (Hillsboro) High Sc | 6:24.61 | Verified |
| 8 | BASHAM, Cadynce | FR | Cuba High School | 6:42.25 | Verified |
| 9 | LAURINE, Elizabeth | JR | Rosati-Kain High School | 6:44.31 | Verified |
| 10 | JONES, Ruby | FR | Pacific High School | 6:59.56 | Verified |
| 11 | HARDCASTLE, Patricia | FR | Cuba High School | 7:08.76 | Verified |
| 12 | VANCE, Sophie | FR | Potosi High School | 7:08.89 | Verified |
| 13 | LANE, Hanna | SR | Pacific High School | 8:02.76 | Verified |