| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Crystal City High School | A | Raftery | Kuchera | Mayes | DeGeare | 1:47.43 | Verified |
| 3 | Grandview-R2 (Hillsboro) High Sc | A | Keim | Maxwell | Walker | Haynes | 1:41.94 | Verified |
| 4 | Potosi High School | A | Sprous | Cross | Kanan | Brabham | 1:38.42 | Verified |
| 5 | Pacific High School | A | Thomas | Magsigay | Reeder | Krug | 1:41.53 | Verified |
| 6 | Cuba High School | A | Laney | Volner | DeClue | West | 1:45.03 | Verified |