| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Potosi High School | A | Randall | Gibson | Gibson | Randall | 2:00.05 | Verified |
| 4 | Grandview-R2 (Hillsboro) High Sc | A | Boker | Morris | Wakeland | Wakeland | 1:53.67 | Verified |
| 5 | Pacific High School | A | Bonner | Murphy | Blackburn | Reeder | 1:57.93 | Verified |
| 6 | Cuba High School | A | Viramontes | Martellaro | Lunsford | West | 2:12.48 | Verified |