| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Potosi High School | A | Sansegraw | Gibson | Coleman | Gibson | 4:46.14 | Verified |
| 4 | Grandview-R2 (Hillsboro) High Sc | A | Wakeland | Boker | Morris | Wakeland | 4:24.90 | Verified |
| 5 | Pacific High School | A | Daniel | Murphy | Reed | Dryer | 4:44.42 | Verified |
| 6 | Cuba High School | A | Black | Weber | Ijames | Viramontes | 5:09.28 | Verified |