| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | STONE, Kaylee | SO | Potosi High School | 36.18 | Verified |
| 4 | WEBER, Bailey | FR | Cuba High School | 32.72 | Verified |
| 5 | LAURINE, Elizabeth | JR | Rosati-Kain High School | 33.14 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HEMPHILL, Ka'Nise | SO | Rosati-Kain High School | 32.39 | Verified |
| 2 | LUNSFORD, Ciara | JR | Cuba High School | 31.98 | Verified |
| 3 | CLONTS, Cierra | JR | Bourbon High School | 31.25 | Verified |
| 4 | POOLE, Abigail | SO | Bourbon High School | 30.49 | Verified |
| 5 | BLACK, Rylee | JR | Cuba High School | 30.84 | Verified |
| 6 | ZABAN, Francesca | JR | Potosi High School | 31.40 | Verified |
| 7 | COLE, Aniyah | JR | Rosati-Kain High School | 32.12 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THURMAN, Lexi | JR | Crystal City High School | 29.90 | Verified |
| 2 | BONNER, Layla | SO | Pacific High School | 29.00 | Verified |
| 3 | LAY, Alexis | JR | Pacific High School | 28.45 | Verified |
| 4 | ENKE, Marisa | SO | Bourbon High School | 27.34 | Verified |
| 5 | MCCLOUD, Samyia | SO | Potosi High School | 27.56 | Verified |
| 6 | MCCLUNE, Danielle | SR | Grandview-R2 (Hillsboro) High Sc | 28.67 | Verified |
| 7 | MURPHY, Ella | SR | Pacific High School | 29.06 | Verified |
| 8 | WIENKE, Elyse | SO | Grandview-R2 (Hillsboro) High Sc | 29.99 | Verified |