| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KANAN, Jaden | SR | Potosi High School | 2:08.02 | Verified |
| 2 | SISK, Ezekiel | JR | Potosi High School | 2:12.92 | Verified |
| 3 | GIBSON, Connor | SR | Potosi High School | 2:13.37 | Verified |
| 4 | PAYNE, Brett | FR | Bourbon High School | 2:13.37 | Verified |
| 5 | STAHL, Cody | SO | Pacific High School | 2:21.39 | Verified |
| 6 | SMITH, Ben | FR | Pacific High School | 2:22.98 | Verified |
| 7 | SAPPINGTON, Elijah | JR | Bourbon High School | 2:24.51 | Verified |
| 8 | WIGGER, Sean | SO | Pacific High School | 2:28.95 | Verified |
| 9 | JONES, Max | FR | Cuba High School | 2:41.06 | Verified |
| 10 | FOX, Tyler | JR | Grandview-R2 (Hillsboro) High Sc | 2:45.81 | Verified |
| 11 | MORALES, Tristan | SR | Crystal City High School | 2:47.88 | Verified |